It can be applied to the soil via drip irrigation, incorporation into the substrate, or as a seed treatment, ensuring contact with the root zone. It is also used in foliar applications to prevent fungal diseases in leaves and fruit. Bacillus subtilis rapidly colonizes the surface of roots and other plant tissues, forming a biological barrier that prevents pathogens from establishing themselves and activates the plant's natural defenses.

It is recommended to apply it preventively or in the early stages of crop development, maintaining adequate humidity conditions that promote the survival and multiplication of the bacteria.