Apple chlorotic leafspot virus (ACLSV) is a single-stranded RNA virus that belongs to the genus Trichovirus, within the family Betaflexiviridae. This virus is common in pome fruit trees and is spread mainly through infected propagating material, such as cuttings and rootstocks. The virus infects the host cells, replicating in the cytoplasm and producing new viral particles that spread through the plant's vascular system. The infection can be asymptomatic or cause variable symptoms depending on the strain of the virus and the susceptibility of the host. In infected cells, the virus travels through the plasmodesmata and moves systematically within the plant. The persistence of the virus in plant material ensures its transmission to new generations of plants.